Preparation and characterization of immobilized glucose-phosphate isomerase
✍ Scribed by L.M. Simon; M. Kotormán; B. Szajáni; L. Boross
- Book ID
- 107888831
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 457 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0141-0229
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## Abstract The immobilization of glucose isomerase (D‐xylose ketol isomerase, EC 5.3.1.5) by covalently bonding to various carriers and by adsorption to ion exchange resins was attempted in order to obtain a stable immobilized enzyme which can be used for continuous isomerization of glucose in a c
Using commercial immobilized glucose isomerase (SWETASEa, Nagase Co.), the effect of substrate protection on enzyme deactivation has been studied in a batch manner. The data analysis was carried out based on Briggs-Haldane kinetics in which enzyme deactivation accompanying the protection of substrat