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Preparation and characterization of 3H-labeled α-bungarotoxin

✍ Scribed by Stephen W. Jones; Williams R. Thompson

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
611 KB
Volume
101
Category
Article
ISSN
0003-2697

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✦ Synopsis

o-Bungarotoxin has been labeled with [3H]pyridoxamine phosphate, by reaction with pyridoxal phosphate followed by reduction with sodium boro["H]hydride. Specific activities of up to 27 Ci/mmol have been obtained. Mono-and dilabeled toxins bind irreversibly to the acetylcholine receptor from Torpedo electroplax, despite a change in isoelectric point from 9.2 for native toxin to 6.2 for dilabeled toxin. The 3H-labeled a-bungarotoxin is usable for over a year.

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