Prenatal diagnosis of tay-sachs disease in cell-free amniotic fluid
✍ Scribed by Christomanou, Helene ;Čáp, Claudia ;Sandhoff, K.
- Publisher
- Springer-Verlag
- Year
- 1978
- Tongue
- English
- Weight
- 273 KB
- Volume
- 56
- Category
- Article
- ISSN
- 1432-1440
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## Abstract Tay‐Sachs disease and normal fetal cerebellar cells were maintained in culture for up to four weeks. Elevated levels of the lysosomal enzymes hexosaminidase B, β‐galactosidase, and acid phosphatase were observed in the Tay‐Sachs cells in long term cultures although elevated lysosomal en
Hexosaminidase (Hex) A, B, and C/S were electrophoretically separated from cultured amniotic fluid cells, fetal brain, and white blood cells. Photographs of cellulose acetate zymograms were evaluated by reflectometric scanning. The usefulness and limitations of this rapid method were shown. Hex A wa