Preliminary phytochemical investigation of wild rice ergot

✍ Scribed by Gregory T. Sinner; Lee C. Schramm

• Publisher: John Wiley and Sons
• Year: 1968
• Tongue: English
• Weight: 287 KB
• Volume: 57
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600570543

✦ Synopsis

A recalibrated pipet was used in preparing the water in methanol standard. A weighed quantity of standard hydrated sodium acetate might be considered as an alternate.

Applicability to Other Types of Sample-The TABLE I-REAGENT NEEDED TO TITRATE SAMPLES SUBJECTED TO VARYING PRETREATMENT, ml. Lyophilized substances-moisture in vials Vials-moisture determination Karl Fischer titration-analysis Methanol-reagent A 0.23 0.24 0.28 0.25 0.23 0.28 0.25 0.27 0.24 B 0.24 0.25 0.30 0.29 0.22 0.24 0.23 0.24 0.30

authors found that a lyophilized B complex containing 10 mg. riboflavin could be titrated to an easily identified end point despite the color in the sample. Other types of sample have not been tested but it would seem that the method is applicable to any parenteral-type vial containing lyophilized or powdered sample low in water content, unless it is deeply colored or contains a substance which interferes with the titration, such as ascorbic acid. Simple vials do not require the preliminary preparation described for the double-chambered vial, but can be titrated directly. TABLE 11-DETERMINATION