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Preliminary crystallographic analyses of the N-terminal lobe of recombinant human serum transferrin

โœ Scribed by Yili Wang; Jie Chen; Yaoguang Luo; Walter D. Funk; Anne B. Mason; Robert C. Woodworth; Ross T.A. MacGillivray; Gary D. Brayer


Book ID
115985783
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
248 KB
Volume
227
Category
Article
ISSN
0022-2836

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19F NMR was utilitized to study human serum transferrin, which is a bilobal protein with a high-affinity metalbinding site in each lobe. The N-terminal lobe of the recombinant protein (residues 1-337 ; hTF/2N) and three single point mutants, H207E, W8Y and W128Y, were expressed in baby hamster kidne