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Preliminary characterization of the acetylcholine receptor in human erythrocytes

✍ Scribed by Huestis, Wray H.

Publisher
Wiley (John Wiley & Sons)
Year
1976
Tongue
English
Weight
477 KB
Volume
4
Category
Article
ISSN
0091-7419

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✦ Synopsis

Abstract

The response of human erythrocytes to cholinergic ligands was studied with an electron spin resonance assay. The membrane response to carbamyl choline was found to be antagonized by atropine and, in the absence of calcium, by tetrodotoxin. Experiments with resealed ghosts showed that the membrane response to carbamyl choline required ATP and calcium. Reductive alkylation of intact cells eliminated the cholinergic response, but the presence of saturating amounts of carbamyl choline protected the putative receptor against inactivation. Affinity labeling was used to demonstrate an apparent molecular weight of 41,000 for the carbamyl choline‐binding species. A lipid vesicle extraction technique was used to induce a specific cation permeability defect in intact cells. Preliminary investigation of this phenomenon is described.

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