Predictive value of Ki-67, p53 protein, and DNA content in the diagnosis of gastric carcinoma

✍ Scribed by Nobuyuki Igarashi; Makoto Takahashi; Haruo Ohkubo; Kousaku Omata; Ryuichi Iida; Shigeru Fujimoto

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 76 KB
• Volume: 86
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/(sici)1097-0142(19991015)86:8<1449::aid-cncr10>3.0.co;2-d

✦ Synopsis

BACKGROUND.

The ability to make a precise preoperative diagnosis is a valuable and effective method in improving the prognosis of patients with gastric carcinoma. The authors examined retrospectively whether preoperative histopathologic analysis with p53 protein, Ki-67 labeling index, and DNA ploidy along with preoperative radiographic and endoscopic findings led to a precise preoperative diagnosis of patients with gastric carcinoma.

METHODS.

Histopathologic analysis of p53 protein, Ki-67 labeling index, and DNA content was performed on formalin fixed, paraffin embedded tissue. Tissue sections from endoscopic and surgically resected specimens were stained immunohistochemically for p53 protein and Ki-67 labeling index, and the cell nuclear DNA content of the surgically resected primary lesion was measured using a microspectrophotometer. These analyses were performed on 16 patients with early gastric carcinoma (EGC) who were diagnosed with advanced gastric carcinoma (AGC) based on the preoperative imaging findings and on 15 patients with AGC who were diagnosed preoperatively with EGC.