Predictive parameters for granulocyte colony-stimulating factor-induced peripheral blood stem cell mobilization

✍ Scribed by Akira Okano; Eishi Ashihara; Chihiro Shimazaki; Hitoji Uchiyama; Tohru Inaba; Kyoko Taniguchi; Taira Maekawa; Masafumi Taniwaki

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 223 KB
• Volume: 23
• Category: Article
• ISSN: 0733-2459
• DOI: 10.1002/jca.20179

✦ Synopsis

Abstract

To improve the selection of donors for allogeneic stem cell transplantation, it is important to identify reliable parameters that predict CD34+‐cell yields after granulocyte‐colony stimulating factor (G‐CSF)‐induced peripheral blood stem cell (PBSC) mobilization. We retrospectively investigated the peripheral blood (PB) kinetics of white blood cells (WBCs), CD34+ cells, matrix metalloproteinases (MMP)‐9 and ‐2, and tissue inhibitors of metalloproteinases (TIMP)‐1 and ‐2 in 15 healthy donors during their treatment with G‐CSF. All donors received 10 μg/kg of recombinant human G‐CSF once a day subcutaneously. Leukapheresis was initiated after 4 days of G‐CSF treatment, and G‐CSF treatment continued until the last day of leukapheresis. WBC and CD34+ cell numbers in the PB rose after 2 and 3 or 4 days of G‐CSF treatment, respectively. The PB CD34+ cell numbers on day 4 correlated weakly with the increase in WBC counts from day 1 to day 2 (R^2^ = 0.254, P = 0.056). There were also positive correlations between the CD34+ cell numbers in the PBSC products on day 4 and the CD34+ cells in the PB on days 1 and 4 (R^2^ = 0.768, P < 0.0001 and R^2^ = 0.816, P < 0.0005, respectively). The MMP‐9 plasma levels on days 1 and 4 also correlated positively with the day 4 circulating CD34+ cell numbers (R^2^ = 0.393, P < 0.05 and R^2^ = 0.406, P = 0.01, respectively). In conclusion, the CD34+ cell numbers in the PB steady state may be a useful parameter selecting allogeneic PBSC donors. J. Clin. Apheresis, 2008. © 2008 Wiley‐Liss, Inc.