## Abstract In reversedβphase liquid chromatography, the retention mechanism of solute has been studied under linearly programmed gradient mobileβphase conditions. The separation of a mixture of four purine compounds (purine, theobromine, theophylline, and caffeine) was considered as a practical ca
Prediction of Retention Time of Solutes under Linear Gradient Elution Conditions in RP-HPLC
β Scribed by Yang Geng-Liang; Tian Bao-Juan; Chen Yi; Wang De-Xian
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 243 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0256-7660
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract The analysis of amino acids presents significant challenges to contemporary analytical separations. The present paper investigates the possibility of retention prediction in hydrophilic interaction chromatography (HILIC) gradient elution based on the analytical solution of the fundament
## Abstract The use of gradient elution in RPβHPLC is increasing. Aqueous buffers, such as phosphate at pH 1.7, with acetonitrile as the organic modifier are particularly advantageous as eluents with linear elution strength (LES). In the isocratic mode, under the experimental conditions used, fairl
The retention behaviour of non-ionic surfactants containing different hydrophobic moiety and various lengths of polar ethylene oxide chain was studied on RP-8 and RP-18 columns using methanol and acetonitrile as organic modifiers. The relative impact of the chromatographic conditions on the retentio
## Abstract The combined effect of temperature, __T__, and organic modifier concentration, Ο, on the retention under gradient conditions in RPLC is studied by considering, both theoretically and experimentally gradients, of Ο at constant __T__ and gradients of __T__ at constant Ο. Two approaches ar