Combined effect of temperature and organic modifier concentration on the retention under single mode gradient conditions in reversed-phase HPLC
✍ Scribed by Adriani Pappa-Louisi; Panos Nikitas; Christiana Zisi; Kostas Papachristos
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 491 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
The combined effect of temperature, T, and organic modifier concentration, φ, on the retention under gradient conditions in RPLC is studied by considering, both theoretically and experimentally gradients, of φ at constant T and gradients of T at constant φ. Two approaches are examined: in the first approach the prediction of the elution time of a sample solute is based on the isocratic/isothermal properties of this solute. The second approach is based on a direct fitting procedure of a proper retention model to 2‐D isocratic/T‐gradient or isothermal/φ‐gradient retention data. These approaches were tested using alkylbenzes in eluting systems modified by ACN. We found that both approaches can give excellent predictions under certain prerequisites. However, the first approach exhibits the notable advantage that it can be used effectively to predict retention times under any kind of φ‐gradients at constant T or T‐gradients at constant φ. The second approach has the advantage that it is relatively simple but its applicability is very restricted since its predictions are satisfactory only if the gradients are of the same kind with those used in the fitting procedure and the conditions lie within those used for fitting.