✦ LIBER ✦

Prediction of diffusion coefficients of plasmids

✍ Scribed by D.M.F. Prazeres

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 118 KB
Volume: 99
Category: Article
ISSN: 0006-3592
DOI: 10.1002/bit.21626

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The ability to predict diffusion coefficients is important in the design, analysis, and operation of plasmid downstream processing operations such as membrane and fixed‐bed chromatography. A correlation is proposed to predict the diffusion coefficient, D, of supercoiled plasmid DNA molecules in dilute solutions on the basis of the molecular weight, M, or size. Experimental data (18 points) collected from the literature confirmed the proposed variation of D with plasmid molecular weight as D ∝ M^−2/3^, for molecules within the 1,800–287,100 base‐pair range. The correlation was able to estimate the available experimental results with an average error of 6.3%. Biotechnol. Bioeng. 2008; 99; 1040–1044. © 2007 Wiley Periodicals, Inc.

📜 SIMILAR VOLUMES

Prediction of diffusion coefficients of

Prediction of diffusion coefficients of proteins

✍ Myo T. Tyn; Todd W. Gusek 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 887 KB

Prediction of diffusion coefficients of

Prediction of diffusion coefficients of proteins

✍ Myo T. Tyn; Todd W. Gusek 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 887 KB

Correlation and prediction of gaseous di

Correlation and prediction of gaseous diffusion coefficients

✍ T. R. Marrero; E. A. Mason 📂 Article 📅 1973 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 663 KB

Prediction of diffusion coefficients for

Prediction of diffusion coefficients for polymer-solvent systems

✍ J. L. Duda; J. S. Vrentas; S. T. Ju; H. T. Liu 📂 Article 📅 1982 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 712 KB

Prediction of mutual diffusion coefficie

Prediction of mutual diffusion coefficients for polymer–solvent systems

✍ J. S. Vrentas; C. M. Vrentas 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 93 KB 👁 3 views

Prediction of binary diffusion coefficie

Prediction of binary diffusion coefficients of solutes in supercritical solvents

✍ Chao-Hong He 📂 Article 📅 1997 🏛 American Institute of Chemical Engineers 🌐 English ⚖ 354 KB 👁 2 views

New correlations, based on the rough hard-sphere theory and an extensive literature database, were developed for the determination of the binary difision coeficients of liquid and solid solutes in supercritical solvents. The correlations were tested to predict 107 solute-solvent systems with good re