Preconcentration of proteins using modified micellar phases of sodium dodecyl sulfate
β Scribed by V. S. Starova; S. A. Kulichenko
- Book ID
- 110212559
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2010
- Tongue
- English
- Weight
- 152 KB
- Volume
- 65
- Category
- Article
- ISSN
- 1061-9348
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Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of prote
## Abstract A microchip gel electrophoresis (MCGE) method with electrokinetic supercharging (EKS, electrokinetic injection with transient isotachophoresis) on a single channel chipΒ was developed for highβsensitive detection of a standard mixture of six proteins (phosphorylase __b__, albumin, ovalbu