Precolumn o -Phthalaldehyde- N -acetyl-L-cysteine Derivatization Followed by RP-HPLC Separation and Fluorescence Detection of Sitagliptin Enantiomers in Rat Plasma

Determination of eflornithine enantiomer

Determination of eflornithine enantiomers in plasma by precolumn derivatization with o-phthalaldehyde-N-acetyl-l-cysteine and liquid chromatography with UV detection

✍ R. Jansson-Löfmark; S. Römsing; E. Albers; M. Ashton 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 363 KB 👁 1 views

## Abstract A bioanalytical method for indirect determination of eflornithine enantiomers in 75 μL human plasma has been developed and validated. l‐ and d‐eflornithine were derivatized with __o__‐phthalaldehyde and __N__‐acetyl‐L‐cysteine to generate diastereomers which were separated on two serial