Determination of eflornithine enantiomers in plasma by precolumn derivatization with o-phthalaldehyde-N-acetyl-l-cysteine and liquid chromatography with UV detection

✍ Scribed by R. Jansson-Löfmark; S. Römsing; E. Albers; M. Ashton

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 363 KB
• Volume: 24
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1361

✦ Synopsis

Abstract

A bioanalytical method for indirect determination of eflornithine enantiomers in 75 μL human plasma has been developed and validated. l‐ and d‐eflornithine were derivatized with o‐phthalaldehyde and N‐acetyl‐L‐cysteine to generate diastereomers which were separated on two serially connected Chromolith Performance columns (RP‐18e 100 × 4.6 mm i.d.) by a isocratic flow followed by a gradient flow for elution of endogenous compounds. The diastereomers were detected with UV (340 nm). The between‐day precisions for L‐ and D‐eflornithine in plasma were 8.4 and 2.3% at 3 μm, 4.0 and 5.1% at 400 μm, and 2.0 and 3.7% at 1000 μm. The lower limit of quantification was determined to be 1.5 μm, at which precision was 14.9 and 9.9% for L‐ and D‐eflornithine, respectively. Copyright © 2009 John Wiley & Sons, Ltd.