Precise quantitation of chloramphenicol acetyl transferase reporter mRNA by competitive polymerase chain reaction
✍ Scribed by Anna Greco; Thierry Massé; Dr. Jean-Jacques Madjar
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 283 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7 M urea, the Cy5‐labeled single‐stran