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Analysis of Promoter Activity by Polymerase Chain Reaction Amplification of Reporter Gene mRNA

✍ Scribed by S. Kastenbauer; A. Wedel; M. Frankenberger; T. Wirth; H.W.L. Ziegler-Heitbrock

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
88 KB
Volume
233
Category
Article
ISSN
0003-2697

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✦ Synopsis

Analysis of Promoter Activity by Polymerase

large intron, that will be spliced out during processing Chain Reaction Amplification of Reporter to mature mRNA. Using a primer, designed to bridge Gene mRNA this intron, mRNA can be selectively detected, and this can be done with the exquisitely high sensitivity of PCR.

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