## Contents A technique for __in vitro__ maturation of oocytes from small ovarian follicles of marmoset monkeys (__Callithrix jacchus__) has been developed. We employed a two‐step culture system for primary follicles (45–85 __μ__m) and a one‐step culture technique for secondary follicles (>85 __μ__
Pre-ovulatory RNA Synthesis in human oocytes of large antral follicles
✍ Scribed by Tesařík, J. ;Kopečny, V. ;Kurilo, L. F.
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 441 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0018-2214
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## Abstract Oocytes were dissected from late, middle, and pre‐antral Graafian follicles from sexually mature mice (6–8 weeks old), cultured in vitro in a chemically defined medium for 18 hours, and scored for meiotic maturation. Between 83–91% of the oocytes removed from antral follicles (300–600 μ
The intranuclear distribution of two (unphosphorylated and hyperphosphorylated) forms of RNA polymerase II (Pol II) was studied in human oocytes from antral follicles using immunogold labeling/electron microscopy. The distribution of Pol II was analyzed relative to the transcriptional state of the o