In vitro maturation of mouse oocytes isolated from late, middle, and pre-antral graafian follicles
✍ Scribed by Erickson, Gregory F. ;Sorensen, Ralph A.
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 233 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Oocytes were dissected from late, middle, and pre‐antral Graafian follicles from sexually mature mice (6–8 weeks old), cultured in vitro in a chemically defined medium for 18 hours, and scored for meiotic maturation. Between 83–91% of the oocytes removed from antral follicles (300–600 μm in diameter) progressed to either metaphase I or metaphase II. In marked contrast, 98% of the oocytes removed from pre‐antral follicles (100–150 μm in diameter) failed to resume meiosis and remained in the germinal vesicle stage. These and other data suggest there is a close correlation between the capacity of the mouse oocyte to resume meiosis and the appearance of the antrum in the developing follicle.