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Practical aspects of ROESY experiments for identification of bound waters in the cyclic tetrasaccharide

✍ Scribed by Kazuo Furihata; Takashi Fujimoto; Ayumi Tsutsui; Tomoya Machinami; Mitsuru Tashiro

Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 142 KB
Volume: 43
Category: Article
ISSN: 0749-1581
DOI: 10.1002/mrc.1702

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✦ Synopsis

ROESY pulse sequences are presented and evaluated to identify bound waters in the cyclic tetrasaccharide. The first experiment incorporated the double-pulsed field gradient spin-echo (DPFGSE) for selective water excitation at the initial portion of the pulse sequence. Although long, shaped pulses were used in DPFGSE to achieve the highly selective excitation of water resonance that is very close to resonances of the cyclic tetrasaccharide, the approach was not effective because of the loss of sensitivity. Concomitant use of long delays and moderate length of shaped pulses in the portion of DPFGSE gained more sensitivity. A simple approach incorporating spin-echo with long delays instead of DPFGSE also afforded a sensitive spectrum. Practical aspects of these ROESY experiments are illustrated using the cyclic tetrasaccharide

cyclo-f→6g-a-D-Glcp-(1→3)-a-D-Glcp-(1→6)-a-D-Glcp-(1→3)-a-D-Glcp-(1→).

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