## Abstract A novel pulse sequence incorporating the double pulsed field gradient spinโecho (DPFGSE) and the gradientโtailored excitation WATERGATE techniques is presented that has particular use for identifying bound waters in ^15^Nโlabeled macromolecules. This sequence, DPFGSEโROESYโHSQC, affords
New pulsed field gradient NMR experiments for the detection of bound water in proteins
โ Scribed by Claudio Dalvit; Ulrich Hommel
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 392 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0925-2738
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This paper presents a software program, the Virtual NMR Spectrometer, for computer simulation of multichannel, multidimensional NMR experiments on user-defined spin systems. The program is capable of reproducing most features of the modern NMR experiment, including homo- and heteronuclear pulse sequ
## Abstract Novel pulse sequences incorporating the double pulsed field gradient spinโecho technique are presented that have particular use in identifying macromolecular bound water. The use of these sequences is illustrated using ribonuclease T~1~. Five amide protons crossโrelaxing with bound wate