Potentiality of the coupling of column liquid chromatography and field desorption mass spectrometry

✍ Scribed by H.-R. Schulten; H.D. Beckey

• Publisher: Elsevier Science
• Year: 1973
• Tongue: English
• Weight: 527 KB
• Volume: 83
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97047-x

✦ Synopsis

The first use of column liquid chromatography in combination with field desorption mass spectrometry is exemplified by the separation and identification of the components of a steroid mixture extracted from rat serum. There are two main reasons for the high-molecular-ion intensities obtained : (1) the smaller transferred energy in the field ionization process compared with other ionization modes increases the chance of deteoting the intact molecular ions; and (2) the samples are not introduced via the commonly used direct introduction system for evaporation, but are applied to the field ion emitter from a solution using the emitter dipping technique. Hence ionization and desorption of the adsorbed molecules can be performed with minimum thermal stress, Potential applications for the coupling of liquid chromatography and field desorption mass spectrometry are discussed, especially in relation to the handling of the sample and the limits of detection.