Potential pyrophosphate formation upon use ofNα-Fmoc-Tyr(PO3H2)-OH in solid-phase peptide synthesis

✍ Scribed by Carlos García-Echeverría

• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 361 KB
• Volume: 2
• Category: Article
• ISSN: 1573-3149
• DOI: 10.1007/bf00128503

✦ Synopsis

A series of peptides related to some of the autophosphorylation sites of the epidermal growth factor receptor has been synthesized on solid phase, using side-chain-unprotected phosphotyrosine. Although the desired peptides could be obtained, this approach was not entirely satisfactory due to a side reaction that gave the pyrophosphate derivative of the target peptide. Under identical coupling conditions, the extension of this side reaction appears to be sequence dependent.