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Evaluation of coupling conditions for the incorporation ofNα-Fmoc-Tyr(PO3H2)-OH in solid-phase peptide synthesis

✍ Scribed by Carlos García-Echeverría

Publisher: Springer Netherlands
Year: 1996
Tongue: English
Weight: 305 KB
Volume: 2
Category: Article
ISSN: 1573-3149
DOI: 10.1007/bf00120001

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✦ Synopsis

In order to minimise the formation of the pyrophosphate derivative of the target peptide when side-chainunprotected phopshotyrosine is used in solid-phase peptide synthesis, this building block can be incorporated using benzotriazolyloxy-tris-(dimethylamino)phosphonium hexafluorophosphate/1-hydroxybenzotriazole/N-methylmorpholine (1:1:2.3) in the presence of a chaotropic salt (0.4 M LiCI in N-methyl-2-pyrrolidinone).

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