Potential- and surface-dependent behaviour of thrombin adsorbed on carbon electrodes

✍ Scribed by Hyacinthe Randriamahazaka; Jean-Maxime Nigretto

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 914 KB
• Volume: 257
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85178-9

✦ Synopsis

A procedure IS described for the duect nnmoblhzatlon of thrombm on carbon paste and solid carbon electrodes with good reproductblhty and improved stablhty for the enzyme Typically, the radlolabelhng of thrombm with lodme-125 indicated that enzyme densities of about 2 x lo-l3 mol cme2 could be achieved at saturation Thus gave a rate of coverage of about 20% of the electrochemical area The catalytic actlvltles and the kmetlc parameters were estimated under forced convection condlttons urlth a specific substrate (S-2238) provided wth chromogemc propertles and dissolved m the solution No maJor changes m K, or V, were found due to adsorption However, increased actlvittes were detected when adsorption was conducted at positwe prepolarlzatlon of the electrode or after potentml cychng m the presence of p-mtroamhne A pattern of interaction with the surface sites of adsorptlon IS proposed