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Characterization of DNA Layers Adsorbed on Glassy Carbon Electrodes

✍ Scribed by María L. Pedano; Lía I. Pietrasanta; Manuel López Teijelo; Gustavo A. Rivas

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
756 KB
Volume
20
Category
Article
ISSN
1040-0397

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✦ Synopsis

Abstract

A DNA layer adsorbed at glassy carbon electrodes (GCE) was characterized by ellipsometry, atomic force microscopy (AFM) and scanning electron microscopy (SEM). The presence of the adsorbed DNA layer on polished glassy carbon electrodes was assessed indirectly by ellipsometric measurements. Ellipsometry was also useful to evaluate the influence of the oxide layer formed on glassy carbon electrodes, either spontaneously or after electrochemical pretreatments, on the DNA adsorption and further electrooxidation process. SEM and AFM images of the electrode surface covered by a thick layer of DNA reveal a nonuniform distribution, leaving channels and islands of the biological material.

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