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Potassium content of single human red cells measured with an electron probe

✍ Scribed by R. Gary Kirk; Miles A. Crenshaw; D. C. Tosteson


Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
700 KB
Volume
84
Category
Article
ISSN
0021-9541

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✦ Synopsis


Abstract

The potassium content of single human red cells was measured with an electron probe. Cells were placed on beryllium discs and coated with a thin layer of dibutyl pthalate to prevent loss of cellular contents. Samples were stable under the electron beam during analysis for more than 15 minutes and could be stored for long periods of time. Primary standards were prepared by loading red cells with varying known amounts of potassium in order to circumvent the corrections for absorption. The X‐ray intensity was found to be directly proportional to the potassium content of the cells.


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Electron probe microanalysis of chemical
✍ C. P. Lechene; C. Bronner; R. G. Kirk πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 669 KB

## Abstract Sodium, potassium, iron and sulfur contents of single human red cells were measured using electron microprobe microanalysis. Three preparative procedures were compared, and the most reliable technique was found to be spraying of cells onto polished pyrolytic graphite by atomization. Pri