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Electron probe microanalysis of chemical elemental content of single human red cells

✍ Scribed by C. P. Lechene; C. Bronner; R. G. Kirk

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
669 KB
Volume
90
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Sodium, potassium, iron and sulfur contents of single human red cells were measured using electron microprobe microanalysis. Three preparative procedures were compared, and the most reliable technique was found to be spraying of cells onto polished pyrolytic graphite by atomization. Primary standards were prepared by adjusting the intracellular electrolyte content of red cells, eliminating the need to correct for X‐ray absorption. Samples were stable under the electron beam during analysis, and could be stored for long periods of time. Strong correlations were found between the X‐ray intensities of iron and sulfur and between potassium and sodium. X‐ray intensities of potassium and sodium were found to be directly proportional to internal ionic content. Large populations of single cells could be analyzed and the distribution of their elemental content studied.

πŸ“œ SIMILAR VOLUMES

Potassium content of single human red ce
Potassium content of single human red cells measured with an electron probe
✍ R. Gary Kirk; Miles A. Crenshaw; D. C. Tosteson πŸ“‚ Article πŸ“… 1974 πŸ› John Wiley and Sons 🌐 English βš– 700 KB

## Abstract The potassium content of single human red cells was measured with an electron probe. Cells were placed on beryllium discs and coated with a thin layer of dibutyl pthalate to prevent loss of cellular contents. Samples were stable under the electron beam during analysis for more than 15 m