Posttranslational regulation of repressible acid phosphatase in yeast

✍ Scribed by Schweingruber, Martin Ernst ;Schweingruber, Anne-Marie

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 314 KB
• Volume: 173
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00268647

✦ Synopsis

On the basis of genetic data it has been suggested that repressible acid phosphatase of Saccharomyces cerevisiae is regulated by a control circuit involving operator-repressor mechanisms (Toh-e et al., 1978). We measured no significant difference in the amount of translatable mRNA of repressed and derepressed cells in the reticulocyte in vitro translation system. We find a 25 fold difference in specific enzyme activity in repressed versus derepressed cells whereas the amount of 35S-methionine labelled enzyme protein as measured by antibody precipitation varies only 2-3 fold. This argues for posttranslational regulation of preexisting inactive acid phosphatase. Minor regulatory effects at the transcriptional or translational level cannot be excluded.