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Comparison of the phosphate-repressible and-constitutive acid phosphatases ofYarrowia lipolytica

✍ Scribed by D.N. Galabova; E.S. Vasileva-Tonkova; M.A. Balasheva


Publisher
Springer
Year
1994
Tongue
English
Weight
213 KB
Volume
10
Category
Article
ISSN
1573-0972

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✦ Synopsis


The phosphate-repressible acid phosphatase from Yawowia lipolytica cells was more thermostable but more sensitive to urea denaturation and to the effect of Triton X-100 than the constitutive counterpart. The K, values of the repressible and constitutive enzymes for p-nitrophenyl phosphate were 3.6 mM and 7.4 mM, respectively. They are judged to be distinct proteins.


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