𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Posttranscriptional regulation of ornithine decarboxylase activity

✍ Scribed by Lori Dircks; Ann Grens; Thelma C. Slezynger; Immo E. Scheffler

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
891 KB
Volume
126
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

We have used a Chinese hamster ovary cell line (DF3) that overproduces ornithine decarboxylase (ODC) to examine various parameters in the cell cycle-dependent regulation of this enzyme. Under a variety of conditions, alterations in the activity of ODC were accompanied by parallel changes in the levels of the protein, as measured by immunologically cross-reactive material (CRM). While putrescine has been known to suppress the induction of ODC, we have found that in DF3 cells 10-4M ornithine completely suppresses ODC activity. We also show that the levels of ODC mRNA are not modulated when the levels of ODC activity and CRM change drastically. The data can be interpreted in terms of models involving either an effect of putrescine on t h e translation of ODC mRNA, or on the activity of a relatively specific protease with ODC as its target.

πŸ“œ SIMILAR VOLUMES

Multihormonal regulation of ornithine de
Multihormonal regulation of ornithine decarboxylase activity in highly differentiated porcine granulosa cells in vitro
✍ Johannes D. Veldhuis; Sharon Sweinberg πŸ“‚ Article πŸ“… 1981 πŸ› John Wiley and Sons 🌐 English βš– 677 KB

## Abstract Purified luteinizing hormone, but not follicle‐stimulating hormone, elicited time‐ and dose‐dependent stimulation of the cytosolic enzyme, ornithine decarboxylase, in highly differentiated, porcine granulosa cells maintained in vitro in chemically defined medium. Enzymic induction was s

Management of polyamine pools and the re
Management of polyamine pools and the regulation of ornithine decarboxylase
✍ Rowland H. Davis πŸ“‚ Article πŸ“… 1990 πŸ› John Wiley and Sons 🌐 English βš– 529 KB

The management of polyamine synthesis and polyamine pools differs fundamentally from that of most other small molecular-weight endproducts. The polyamines are vital to growth and important cellular functions, but they are toxic in excess. I argue here that their multivaient cationic character, leadi

Consequences of aberrant ornithine decar
Consequences of aberrant ornithine decarboxylase regulation in rat hepatoma cells
✍ Margaret E. Tome; Steven M. Fiser; Eugene W. Gerner πŸ“‚ Article πŸ“… 1994 πŸ› John Wiley and Sons 🌐 English βš– 757 KB

Arizona 85724 DH23A cells, an a-difluoromethylornithine (DFMO)-resistant variant of rat hepatoma tissue culture cells (HTC), contain high levels of very stable ornithine decarboxylase (ODC). In the absence of DFMO, the high ODC activity results in a large accumulation of endogenous putrescine. Conco