Postcolumn co-immobilized leucine dehydrogenase-NADH oxidase reactor for the determination of branched-chain amino acids by high-performance liquid chromatography with chemiluminescence detection

Determination of branched-chain L-amino

Determination of branched-chain L-amino acids by flow-injection analysis with co-immobilized leucine dehydrogenase/NADH oxidase and chemiluminescence detection

✍ Nobutoshi Kiba; Akira Kato; Motohisa Furusawa 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 530 KB

A method for the determination of branched-chain L-amino acids in plasma is described. Leucine dehydrogenase and NADH oxidase were co-immobilized on aminated poly(viny1 alcohol) beads and incorporated in a flow-injection system with chemiluminescence detection. 20 ~1 sample solution was injected int