Determination of branched-chain L-amino acids by flow-injection analysis with co-immobilized leucine dehydrogenase/NADH oxidase and chemiluminescence detection

✍ Scribed by Nobutoshi Kiba; Akira Kato; Motohisa Furusawa

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 530 KB
• Volume: 311
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00146-q

✦ Synopsis

A method for the determination of branched-chain L-amino acids in plasma is described. Leucine dehydrogenase and NADH oxidase were co-immobilized on aminated poly(viny1 alcohol) beads and incorporated in a flow-injection system with chemiluminescence detection. 20 ~1 sample solution was injected into the system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(II1) reaction. The calibration graph was linear for 5 X lo-'-6 X 10e4 M; the detection limit (signal-to-noise ratio = 3) was 3 X lo-' M and the sample throughput was 15 h-' without carryover.