Possible use of similar framework region amino acid sequences between human and mouse immunoglobulins for humanizing mouse antibodies
β Scribed by Tai Te Wu; Elvin A. Kabat
- Book ID
- 118384491
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 738 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0161-5890
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Monoclonal antibodies (MAbs) were generated by immunizing mice with a truncated recombinant protein corresponding to the immunodominant region (residues 1-120) of hepatitis C virus (HCV) nucleocapsid protein. The specific recognition by either human sera or mouse monoclonal antibodies of overlapping
Materials and methods ### 2.1 Immunological reagents and buffers Anti-C1 q was supplied by Dako (Copenhagen, Denmark). All other antibodies and conjugates were made in our laboratory. Isotonic Verona1 buffer of pH 7.3 was prepared with 1 mM MgCI2 and 1 mM CaCI2.