✦ LIBER ✦
C1q binding to chimeric monoclonal IgG3 antibodies consisting of mouse variable regions and human constant regions with shortened hinge containing 15 to 47 amino acids
✍ Scribed by Inger Sandlie; Audun Aase; Christina Westby; Terje E. Michaelsen
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 708 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Materials and methods
2.1 Immunological reagents and buffers
Anti-C1 q was supplied by Dako (Copenhagen, Denmark). All other antibodies and conjugates were made in our laboratory. Isotonic Verona1 buffer of pH 7.3 was prepared with 1 mM MgCI2 and 1 mM CaCI2.