Possible role of phospholipase C in the induction of Ca2+-paradox in rat heart
β Scribed by Sujata Persad; Alena Vrbanova; Johanna T. A. Meij; Vincenzo Panagia; Naranjan S. Dhalla
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 912 KB
- Volume
- 121
- Category
- Article
- ISSN
- 0300-8177
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It is demonstrated a fast and significant depression in the sarcolemmal (Na,K)-ATPase activity that occurs as early as 25 sec after the onset of Ca2+ depletion, and participates in the development of Ca(2+)-paradox in the rat heart. Pretreatment of the animals with 7-oxo-prostacyclin (PGI2) 24-48 h
The expression of regucalcin, a regulatory protein of Ca(2+) signaling, and its effect on Ca(2+) pump activity in the microsomes (sarcoplasmic reticulum) of rat heart muscle was investigated. The expression of regucalcin mRNA was demonstrated by reverse transcription-polymerase chain reaction (RT-PC