Molecular cloning and nucleotide sequenc
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Akiyama, Masahiro ;Horiuchi, Takashi ;Sekiguchi, Mutsuo
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Article
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1987
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Springer
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English
⚖ 834 KB
The Escherichia coli mutator gene mutT, which causes A:T----C:G transversion, was cloned in pBR 322. mutT+ plasmids carry a 0.9 kb PvuII DNA fragment derived from the E. coli chromosome. Specific labelling of plasmid-encoded proteins by the maxicell method revealed that mutT codes for a polypeptide