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Molecular cloning and nucleotide sequence of the mutT mutator of Escherichia coli that causes A:T to C:G transversion

✍ Scribed by Akiyama, Masahiro ;Horiuchi, Takashi ;Sekiguchi, Mutsuo

Publisher: Springer
Year: 1987
Tongue: English
Weight: 834 KB
Volume: 206
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00326530

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✦ Synopsis

The Escherichia coli mutator gene mutT, which causes A:T----C:G transversion, was cloned in pBR 322. mutT+ plasmids carry a 0.9 kb PvuII DNA fragment derived from the E. coli chromosome. Specific labelling of plasmid-encoded proteins by the maxicell method revealed that mutT codes for a polypeptide of about 15,000 daltons. The protein was overproduced when the mutT gene was placed under the control of the lac regulatory region on a multicopy runaway plasmid. The nucleotide sequence of the mutT gene was determined by the dideoxy method.

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