✦ LIBER ✦

Possibilités de Dosage Spectrophotométrique Direct de la Vitamine D2 en Solution Pure et en Présence de Sels Calciques

✍ Scribed by V. Lasareff; E. V. Rouir

Publisher: Wiley (John Wiley & Sons)
Year: 2010
Weight: 327 KB
Volume: 56
Category: Article
ISSN: 0037-9646
DOI: 10.1002/bscb.19470560910

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✦ Synopsis

Abstract

The sensitivity of a direct spectrophotometric determination of vitamin D~2~ (calciferol), based on its ultra‐violet absorption, proved to be 1 microgram/ml., or better. Compared with the current colorimetric techniques, the ultra‐violet method is capable of a much higher accuracy.‐The ultra‐violet absorption spectrum of calciferol remains unchanged in the presence of calcium phosphate or gluconate. The latter compounds show only a slight continuous absorption in the same spectral range. Consequently, the ultra‐violet determination of calciferol can be performed without previous elimination of calcium salts.

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## Abstract The spectrophotometric determination of traces of Ce^4+^ and Ti^3+^ in the presence of respectively large excesses of Ce^3+^ (≤ 10^–1^ M) and Ti^4+^ (≤ 10^−1^ M), is described. Indirect methods are elaborated for both ions. The reaction of Ce^4+^ with an excess of Fe^2+^ gives an equiv