Positive and negative ion 252Cf plasma desorption mass spectrometry of polar agrochemical metabolites

✍ Scribed by Guenter Allmaier; Erich R. Schmid; Peter Roepstoff

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 439 KB
• Volume: 19
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200190205

✦ Synopsis

The use of 2s2Cf plasma desorption (PD) mass spectrometry for the identification of highly polar agrochemical metabolites is illustrated with metabolites of azinphos-methyl, ethyl, demeton-S-methyl and demeton-S-methyl sulphoxide. Three different classes of metabolites were used, namely the monosodium salt of organophosphorus acid ester, the glycoside and the sodium salt of sulphonic acid. Positive and negative ion mass spectra were recorded for different sample preparation techniques. The electrospray method and nitrocellulose adsorption with sample application by spindrying with and without a rinsing step was applied and evaluated. PD mass spectrometry has proved to be useful in providing fast information on the molecular weight of all three classes of metabolites, but the method has failed to give fragment ions. The best result in terms of sensitivity and low background were obtained using the nitrocellulose spin-drying method without a washing step. It has been found to be a very valuable technique for the rapid identification of non-volatile or thermally labile agrochemical metabolites after highperformance liquid chromatographic isolation from plants.