𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Positional and Temporal Accuracy of Single Molecule Tracking

✍ Scribed by Jörg Enderlein

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
165 KB
Volume
1
Category
Article
ISSN
1438-5163

No coin nor oath required. For personal study only.

✦ Synopsis

In a recent paper, a new method for tracking fluorescing single molecules that diffuse within bilipid layers or cell membranes was presented and its efficiency theoretically studied. In the present paper, two questions concerning this method are addressed: First, the fundamental question of how accurately can the spatial position of the tracked molecule be determined? Second, is it possible to extract temporal information about e.g. the reaction dynamics of a single fluorescence-labeled enzyme from the signal that is measured during single molecule tracking? The answers to these questions are important when estimating the usefulness of the proposed tracking method as an alternative to conventional single molecule tracking with wide field fluorescence microscopes.

📜 SIMILAR VOLUMES

Single Protein Molecules Visualized and
Single Protein Molecules Visualized and Tracked in the Interior of Eukaryotic Cells
✍ Ulrich Kubitscheck 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 74 KB 👁 2 views

In the last few years the visualization and tracking of single fluorescent proteins, nanometer-sized RNP particles and viruses within the cellular interior was accomplished. This became feasible by use of photostable fluorescent dyes, extremely low probe concentrations, and wide-field fluorescence m