Single Protein Molecules Visualized and Tracked in the Interior of Eukaryotic Cells

✍ Scribed by Ulrich Kubitscheck

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 74 KB
• Volume: 3
• Category: Article
• ISSN: 1438-5163
• DOI: 10.1002/1438-5171(200211)3:5/6<267::aid-simo267>3.0.co;2-x

✦ Synopsis

In the last few years the visualization and tracking of single fluorescent proteins, nanometer-sized RNP particles and viruses within the cellular interior was accomplished. This became feasible by use of photostable fluorescent dyes, extremely low probe concentrations, and wide-field fluorescence microscopic setups equipped with sensitive slow-scan or intensified CCD cameras. This paper reviews the results of the studies performed so far, discusses potential problems and gives an outlook on future applications.