To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat
Pores in the outer membrane of Escherichia coli K12
โ Scribed by van Alphen, Wim ;van Selm, Nelke ;Lugtenberg, Ben
- Publisher
- Springer
- Year
- 1978
- Tongue
- English
- Weight
- 868 KB
- Volume
- 159
- Category
- Article
- ISSN
- 0026-8925
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The gene ompA encodes a major outer membrane protein of Escherichia coli. Localized mutagenesis of the part of the gene corresponding to the 21-residue signal sequence and the first 45 residues of the protein resulted in alterations which caused cell lysis when expressed. DNA sequence analyses revea
The outer membranes of Escherichia coli and Salmonella typhimurium contain several proteins that have been shown to produce rather large channels for diffusion [ 11 . One class, called "porins," has apparent molecular weights in the range of 34-37,000, and, when reconstituted into phospholipid-lipo