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Pores in the outer membrane of Escherichia coli K12. Involvement of proteins b and c in the permeation of cephaloridine and ampicillin

✍ Scribed by Wim van Alphen; Ria van Boxtel; Nelke van Selm; Ben Lugtenberg

Book ID
109310067
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
303 KB
Volume
3
Category
Article
ISSN
0378-1097

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Gene encoding a hybrid OmpF-PhoE pore pr
Gene encoding a hybrid OmpF-PhoE pore protein in the outer membrane of Escherichia coli K12
✍ Tommassen, Jan ;Pugsley, Anthony P. ;Korteland, Jaap ;Verbakel, John ;Lugtenberg πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 685 KB

To study the structure-function relationship of outer membrane pore proteins of E. coli K12, a hybrid gene was constructed in which the DNA encoding amino acid residues 2-73 of the mature PhoE protein is replaced by the homologous part of the related ompF gene. The product of this gene is incorporat