✦ LIBER ✦

Pore-Forming Proteins with Built-in Triggers and Switches

✍ Scribed by H. Bayley

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 845 KB
Volume: 23
Category: Article
ISSN: 0045-2068
DOI: 10.1006/bioo.1995.1023

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✦ Synopsis

The self-assembling, pore-forming protein (\alpha)-hemolysin is a monomeri:, 293-amino-acid, water-soluble polypeptide that forms heptameric pores of 1 - to 2 -nm internal diameter in lipid bilayers. By genetic engineering and targeted chemical modification, 've have produced (\alpha)-hemolysins in which pore-forming activity can be triggered or switched on and off by biochemical, chemical, or physical stimuli. These remodeled molecules include proteaseactivated pores, metal-regulated pores, pores that are activated by chemical alkylation, and pores that are turned on with light. Engineered (\alpha)-hemolysins have potential applications that include acting as components of sensors for various analytes, mediating the zontrolled release of drugs and forming building blocks for agents that selectivity damage malignant cells. 1995 Academic Press, Inc.

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