Expression of mRNAs for pore-forming protein and two serine esterases in murine primary and cloned effector lymphocytes
✍ Scribed by Sanjay V. Jaog; Chau-Ching Liu; Byoung S. Kwon; William R. Clark; John D.-E Young
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 762 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
The cDNAs encoding several proteins present in the granules of cytolytic effector lymphocytes have now been cloned. These include the cytolytic pore-forming protein (PFP) or perforin, and at least six serine esterases (SE), also called granzymes. The cDNA probes for PFP, SE-1, and SE-2 are used here to study the expression of these proteins in murine primary effector lymphocytes. Among the stimuli effective in inducing the expression of PFP, SE-1, and SE-2 were recombinant interleukin-2, the lectin concanavalin A in the presence. of phorbol esters, and allogeneic cells in mixed lymphocyte cultures. Some correlation was seen between the levels of PFP and SE mRNAs and cytotoxicity measured in a standard "Cr release assay. We also examined a panel of 13 cloned cytotoxic T lymphocyte (CTL) lines and found that mRNAs for PFP and SE-2 were expressed in all CTL lines, including some that were previously considered not to produce. PFP. Twelve of the 13 CTL lines also proved to possess the mRNA for SE-1. One thymoma cell line, TIMI.4, did not express mRNA for PFP, although it expressed mRNA for SE-I and SE-2.