Pombe: A gene-finding and exon-intron structure prediction system for fission yeast

✍ Scribed by Chen, Ting; Zhang, Michael Q.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 148 KB
• Volume: 14
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/(sici)1097-0061(19980615)14:8<701::aid-yea247>3.0.co;2-#

✦ Synopsis

A special program developed by the authors, called Pombe, identifies protein coding regions in the Schizosaccharomyces pombe genome. Linear discriminant analysis was applied to predict 5 -terminal, internal, 3 -terminal exons (coding-exon) and introns. The accuracy of the prediction was tested by cross verifications. The sensitivity, specificity and correlation coefficient for the internal exon prediction were 98•5%, 99•9% and 98•3% respectively at the nucleotide level. Open reading frames were studied and used to predict intron-less genes: 99•0% of such genes were identified with correct stopping sites. The gene structure was determined by dynamic programming and the prediction achieved 97•0% correlation coefficient at the nucleotide level.