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Isolation and characterization of temperature-sensitive mutations in the gene (rpb3 ) for subunit 3 of RNA polymerase II in the fission yeast Schizosaccharomyces pombe

โœ Scribed by Mitobe, J. ;Mitsuzawa, H. ;Yasui, K. ;Ishihama, A.


Publisher
Springer
Year
1999
Tongue
English
Weight
564 KB
Volume
262
Category
Article
ISSN
0026-8925

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Linker-insertion mutagenesis was used to isolate mutations in the Saccharomyces cerevisiae gene encoding the largest subunit of RNA polymerase II (RPO21, also called RPB1). The mutant rpo21 alleles carried on a plamid were introduced into a haploid yeast strain that conditionally expresses RPO21 fro