✦ LIBER ✦

Polysomes from cultured muscle cells: The cell-free synthesis of myosin

✍ Scribed by Katherine Tepperman; Francine Essien; Stuart M. Heywood

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 628 KB
Volume: 86
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040860312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The results reported here have shown that there are significant differences between polysome patterns obtained from cultured cells and from freshly isolated muscle tissue. Polysomes from embryonic homogenates show different patterns with different levels of myosin synthesis, but this does not appear to be the case with cultured cells. Experiments utilizing cell‐free protein synthesizing systems indicate that the polysomes isolated from myoblast cultures can synthesize myosin at levels similar to those obtained from myotube cultures, suggesting that the myoblasts contain significant amounts of the messenger RNA for myosin. In contrast, the polysomes isolated from BrdUrd‐inhibited cultures synthesize a comparatively low level of myosin. These findings illustrate a significant difference between myoblasts and BrdUrd‐inhibited cells.

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