𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Culture of quiescent arterial smooth muscle cells in a defined serum-free medium

✍ Scribed by Peter Libby; Kathleen V. O'Brien

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
804 KB
Volume
115
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

An ideal medium for metabolic studies would maintain cultured vascular smooth muscle cells in a quiescent, viable state, as they are in normal arteries in vivo, and would be chemically defined so that the concentrations of hormones and nutrients could be manipulated precisely. In unsupplemented serum-free media these cultures lose protein and DNA, indicating impaired viability. Addition of maximally effective concentrations of insulin (10" M) and transferrin (5 Fg/ml) prevents loss of DNA and produces near neutral protein balance. Further addition of ascorbic acid (10" M) actually promotes net gain of protein with little or no increase in DNA. Ascorbate consistently increased noncollagen protein synthesis by cultured aortic smooth muscle cells. This novel action of t h e vitamin did not require insulin but was additive to the effect of this hormone, and was produced by isoascorbate, but not by a variety of other reducing agents. Thus, vascular smooth muscle cells can be maintained in a quiescent but noncatabolic state in

πŸ“œ SIMILAR VOLUMES

An elastinolytic enzyme detected in the
An elastinolytic enzyme detected in the culture medium of human arterial smooth muscle cells
✍ Yoshikatsu Okada; Shogo Katsuda; Yasunori Okada; Isao Nakanishi πŸ“‚ Article πŸ“… 1993 πŸ› Elsevier Science 🌐 English βš– 418 KB

## Abstract The culture medium of human arterial smooth muscle cells exhibits an elastinolytic activity with 68 and 64 kDa on elastin substrate gels. The enzymatic activities are inhibited by ethylenediamine tetraacetic acid, a metalloproteinase inhibitor, but not by other inhibitors of serine, cys

Cell cycle dependent gene expression in
Cell cycle dependent gene expression in quiescent stimulated and asynchronously cycling arterial smooth muscle cells in culture
✍ Michel Campan; Claude Desgranges; Alain-Pierre Gadeau; Dominique Millet; Francis πŸ“‚ Article πŸ“… 1992 πŸ› John Wiley and Sons 🌐 English βš– 892 KB

## Abstract The expression of a set of cell cycle dependent (CCD) genes (c‐fos, c‐myc, ornithine decarboxylase (ODC), and thymidine kinase (TK)) was comparatively studied in cultured arterial smooth muscle cells (SMC) during exit from quiescence and exponential proliferation. These genes, which wer

A comparison of protein synthetic patter
A comparison of protein synthetic patterns of MDCK cells grown in serum and hormonally defined serum-free medium
✍ Douglas M. Jefferson; Chia-Ping H. Yang; Melanie H. Cobb; Walter N. Scott πŸ“‚ Article πŸ“… 1985 πŸ› John Wiley and Sons 🌐 English βš– 554 KB

The development of hormonally defined serum-free media (HDM) for the culture of mammalian cells has allowed the study of specific cellular functions in a totally defined environment. Recently, several reports have indicated that there are differences in t h e basic cellular physiology of cells cultu

Induction of cell cycle-dependent genes
Induction of cell cycle-dependent genes during cell cycle progression of arterial smooth muscle cells in culture
✍ Alain-Pierre Gadeau; Michel Campan; Claude Desgranges πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 English βš– 626 KB

## Abstract Serum stimulation of arterial smooth muscle cells in culture induces a progression through the cell cycle and cell proliferation. Most genes previously described as cell cycle‐dependent in various cell types also demonstrate a cell cycle‐dependent expression in arterial smooth muscle ce