Polymorphisms within theHLA-DRw6haplotype

✍ Scribed by Marnix L. Bosch; Annemarie Termijtelen; René Gerrets; Geziena M. T. Schreuder; Ronald E. Bontrop; Marius J. Giphart

• Book ID: 104770942
• Publisher: Springer-Verlag
• Year: 1985
• Tongue: English
• Weight: 754 KB
• Volume: 22
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00430591

✦ Synopsis

We have analyzed the HLA class II gene products from HLA-DRw6 homozygous cells. Epstein-Barr virus-transformed B-cell lines were internally labeled with [35S]-methionine. An NP-40 lysate of the cells was subjected to immunoprecipitation, first with a DRw52-1ike-specific monoclonal antibody and subsequently with a DR-specific framework antibody. The DR regionencoded gene products were analyzed by one-dimensional gel isoelectric focusing and two-dimensional gel electrophoresis. It is shown that DRw6 homozygous cell lines contain at least two nonalMic DRp chains, one carrying a DRw52 determinant and one DRw52-negative population. Both chains appear to be polymorphic between the cellularly defined subtypes of DRw6. The determinant responsible for the differential mixed lymphocyte culture reactivity of Dwl 8 and Dwl9 cells resides on the DRw52-positive population, whereas the Dw6-Dw9 differences are attributed to determinants on both populations of DR light chains. The Dwl6-derived DRw52 ÷ chain much resembles the Dwl8 DRw52 + light chain whereas there is a clear-cut difference between these two subtypes in the DRw52-population. We conclude that, for DRw6 homozygous cells, the cellularly recognized D determinants are probably located on DRencoded molecules, both DRw52 + and DRw52-, and that charge shift of these chains is at least partly responsible for differential recognition of these cells in mixed lymphocyte cultures.